Clinical Neuroscience and Neuropathology
نویسندگان
چکیده
DNA methylation is important for controlling gene expression and is catalyzed by DNA methyltransferase (Dnmt1) an enzyme abundant in brain. We recently demonstrated that mice expressing reduced levels of Dnmt1 are protected from cerebral ischemia. Here, we used the cre/loxP system to produce conditional mutants that lack Dnmt 1 in postmitotic neurons of the postnatal brain. We demonstrate that animals heterozygous for the conditional allele (Dnmt11lox/) have signi®cantly smaller infarcts following 1 h middle cerebral artery occlusion/reperfusion compared to their wildtype litters. Surprisingly, mice with a deletion of Dnmt1 in post-mitotic neurons (Dnmt1) were not protected. In conclusion, we demonstrate that reduced levels of Dnmt1, but not its absence, in post-mitotic neurons protect from ischemic brain injury. NeuroReport 12:3763±3766 & 2001 Lippincott Williams & Wilkins.
منابع مشابه
Clinical Neuroscience and Neuropathology
Department of Psychology, Columbia University, New York, NY 10027; Behavioral and Evolutionary Neuroscience Laboratory, Alaskan Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska Fairbanks, P.O. Box 757000, Fairbanks, AK 99775; Department of Psychology, Barnard College, New York, NY 10027; Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Col...
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